Plasmid_Backbone

Part:BBa_K4215000

Designed by: Carmen Resnick   Group: iGEM22_UOregon   (2022-10-10)


pSEVA341

pSEVA341 is compiled with a chloramphenicol antibiotic resistance, pRO1600/ColE1 origin from pUCP24 with a high copy number (~25-40), and an MSC. This backbone is golden-gate compatible with BsaI. The promoter region is designed to have a high transcription rates and the ribosome binding site is designed to have a high translation rate to produce high levels of protein from the inserted gene. The RBS is consistent with the RBS found in the riboJ-RBS part (BBa_K4215001), which allows for controlled bicistronic gene expression.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal XbaI site found at 3132
    Illegal SpeI site found at 3194
    Illegal PstI site found at 3144
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal SpeI site found at 3194
    Illegal PstI site found at 3144
    Illegal NotI site found at 3162
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal BamHI site found at 3126
  • 23
    INCOMPATIBLE WITH RFC[23]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal XbaI site found at 3132
    Illegal SpeI site found at 3194
    Illegal PstI site found at 3144
  • 25
    INCOMPATIBLE WITH RFC[25]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal XbaI site found at 3132
    Illegal SpeI site found at 3194
    Illegal PstI site found at 3144
    Illegal NgoMIV site found at 819
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal BsaI site found at 3220
    Illegal BsaI.rc site found at 3085


[edit]
Categories
Parameters
None